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MB Sample ID: SA201232
Local Sample ID: | 20210522-CH-6 |
Subject ID: | SU002182 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP002188 |
Sampleprep Summary: | 100-130 mg liver tissue samples were weighted, 1.2 mL of 80% ice-cold aqueous methanol, which contains 0.001 mg/mL internal standard, and approximately 1 g of glass beads (1.0 mm i.d.) was added into a 2 mL screw-cap plastic microvial. The Mini-BeadBeater-16 (Biospec Products, USA) was used for tissue disruption. Samples were homogenized 3 times for 2 min each, with cooling procedure in between, centrifuged at 20,000 g for 10 min at 4 °C, and the supernatants were mixed with 800 μL of ice-cold acetonitrile and centrifuged again for protein precipitation. The supernatant was filtered through a 0.22 µm organic phase membrane, blown dry in nitrogen, reconstituted in 100 µL of water and transferred to vials for metabolomics assay. |