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MB Sample ID: SA204111
Local Sample ID: | 156ade_side |
Subject ID: | SU002205 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP002211 |
Sampleprep Summary: | Frozen tissue samples were thawed on ice. Each tissue sample was cut into small pieces and accurately weighed. One mL of precooled 80% methanol was added to 100 mg of tissue and the sample mixture was homogenized (60 Hz, 90 seconds). In a tissue homogenizer with precooled module, followed by vortex-mixing for 1 min and incubation at -80 °C for 4 h to allow for sufficient metabolite extraction. Subsequently, the samples were centrifuged at 14,000 × g for 10 min at 4 °C, and the supernatant was collected and dried under vacuum. Sample blanks were prepared using water following the same experimental procedure. Before analysis, dried metabolites were reconstituted with 100 μL LC-MS grade water, vortex-mixed, and centrifuged at 14,000 × g for 10 min to remove insoluble particles prior to UPLC-HRMS analysis. Quality control (QC) samples were prepared by pooling a small volume from samples with enough volume. |