Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA204434

Local Sample ID:	ybtQ-0-2S
Subject ID:	SU002213
Subject Type:	Bacteria
Subject Species:	Escherichia coli
Taxonomy ID:	562

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MS

Sample Preparation:

Sampleprep ID:	SP002219
Sampleprep Summary:	Siderophores were extracted as previously described. Briefly, 12μL 0.1M ferric chloride was mixed with 2 mL of cell supernatant. After incubating at room temperature for 15 minutes, the precipitate was removed by centrifugation at 20000 × g for 15 min at 4 °C. The supernatant was added to an SPE plate (Waters, Oasis HLB) and washed with 0.5 mL 5% methanol, and then eluted with 0.5 mL 100% methanol to obtain the siderophores. LC/MS analysis was performed using 5μL aliquots.

Sampleprep ID:

SP002219

Sampleprep Summary:

Siderophores were extracted as previously described. Briefly, 12μL 0.1M ferric chloride was mixed with 2 mL of cell supernatant. After incubating at room temperature for 15 minutes, the precipitate was removed by centrifugation at 20000 × g for 15 min at 4 °C. The supernatant was added to an SPE plate (Waters, Oasis HLB) and washed with 0.5 mL 5% methanol, and then eluted with 0.5 mL 100% methanol to obtain the siderophores. LC/MS analysis was performed using 5μL aliquots.