Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA205220

Local Sample ID:	1uM_stdmix_run2
Subject ID:	SU002221
Subject Type:	Synthetic sample

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Sample Preparation:

Sampleprep ID:	SP002227
Sampleprep Summary:	To improve CE peak shape and support electrophoretic focusing, a sample diluent consisting of 133 mM ammonium acetate, and 0.1% formic acid was prepared.13 This sample diluent was spiked with 1 µM 13C phenylalanine, 3 µM 13C6 arginine and 0.8 µM 13C methionine D3 as internal standards. Calibration mixtures were prepared from serial dilution of stock standard solutions using the spiked sample diluent in a 1:4 ratio. Each calibration standard was analyzed twice to yield calibration curves, calculate figures of merit, and perform metabolite quantification.

Sampleprep ID:

SP002227

Sampleprep Summary:

To improve CE peak shape and support electrophoretic focusing, a sample diluent consisting of 133 mM ammonium acetate, and 0.1% formic acid was prepared.13 This sample diluent was spiked with 1 µM 13C phenylalanine, 3 µM 13C6 arginine and 0.8 µM 13C methionine D3 as internal standards. Calibration mixtures were prepared from serial dilution of stock standard solutions using the spiked sample diluent in a 1:4 ratio. Each calibration standard was analyzed twice to yield calibration curves, calculate figures of merit, and perform metabolite quantification.