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MB Sample ID: SA205768
| Local Sample ID: | OI1_D6_S1_Medium |
| Subject ID: | SU002228 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP002234 |
| Sampleprep Summary: | Both blank and conditioned media samples were subjected to a protein-precipitation procedure and dried under vacuum. Prior to NMR analysis, dried media samples were resuspended in 100 mM phosphate buffer at pH 7.4 (prepared in D2O) containing 0.1 mM 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid (TSP in D2O, Sigma-Aldrich 293040), homogenised and transferred to 5 mm NMR tubes. |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 100 mM phosphate buffer at pH 7.4 (prepared in D2O) containing 0.1 mM 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid (TSP in D2O, Sigma-Aldrich 293040) |
