Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA205781

Local Sample ID:	shchrebp HG-3
Subject ID:	SU002230
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090

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MS

Sample Preparation:

Sampleprep ID:	SP002236
Sampleprep Summary:	Mitochondria were isolated from mouse podocytes using Mitochondria Isolation Kit (Thermo Fisher). Mitochondria were pelleted by centrifugation at 3000x g for 15 minutes at 4°C, flash-frozen and stored at -80°C until use. 200 μL of ethanol containing 1% 10 mM butylated hydroxytoluene in methanol, and 2% Avanti SPLASH® LIPIDOMIX® Mass Spec Standards, pre-cooled to -80°C, was added to each mitochondria sample. The tubes were vortexed for 10 min, placed on ice for 10 min, then centrifuged at 4°C for 10 min at 17,000 x g. The supernatants were then collected for LC-MS analysis.

Sampleprep ID:

SP002236

Sampleprep Summary:

Mitochondria were isolated from mouse podocytes using Mitochondria Isolation Kit (Thermo Fisher). Mitochondria were pelleted by centrifugation at 3000x g for 15 minutes at 4°C, flash-frozen and stored at -80°C until use. 200 μL of ethanol containing 1% 10 mM butylated hydroxytoluene in methanol, and 2% Avanti SPLASH® LIPIDOMIX® Mass Spec Standards, pre-cooled to -80°C, was added to each mitochondria sample. The tubes were vortexed for 10 min, placed on ice for 10 min, then centrifuged at 4°C for 10 min at 17,000 x g. The supernatants were then collected for LC-MS analysis.