Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA208748

Local Sample ID:	M17_Negative
Subject ID:	SU002255
Subject Type:	Plant
Subject Species:	Oryza sativa

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Sample Preparation:

Sampleprep ID:	SP002261
Sampleprep Summary:	Extraction of rice samples followed the ultrasonically assisted extraction (UAE) procedure with slight adjustments (Xiao et al., 2018). An A&D HR-120 research-grade analytical balance (A&D, Tokyo, Japan), a Universal 320 centrifuge (Hettich, Georgia, USA) and an Elmasonic 80H ultrasonic bath (Elma Schmidbauer, Singen, Germany) were used for sample extraction. First, each sample was sieved through a 100-m sieve, and then 500  0.1 mg of each homogenized sample was inserted into a 15-mL falcon tube. Afterward, 5 mL of solvent containing ultra-pure water and methanol (1/1, v/v) was added to the tube, and the sample was sonicated for 30 min at 40C and then centrifuged at 6080 x g. The supernatant was filtered through a 0.22-m nylon filter. Consequently, 1 mL of filtered solution was transferred into a dark vial and analyzed by UHPLC-Q-Orbitrap-HRMS as described previously. Procedure blanks consisting of water and methanol (1/1, v/v) were injected after each analyzed sample for background interference removal. Two standard calibrated solutions, Pierce LTQ Velos ESI Positive Ion and Pierce LTQ Velos ESI Negative Ion, were used at the beginning, at the end of each run and after each batch of fifteen analyzed samples.

Sampleprep ID:

SP002261

Sampleprep Summary:

Extraction of rice samples followed the ultrasonically assisted extraction (UAE) procedure with slight adjustments (Xiao et al., 2018). An A&D HR-120 research-grade analytical balance (A&D, Tokyo, Japan), a Universal 320 centrifuge (Hettich, Georgia, USA) and an Elmasonic 80H ultrasonic bath (Elma Schmidbauer, Singen, Germany) were used for sample extraction. First, each sample was sieved through a 100-m sieve, and then 500  0.1 mg of each homogenized sample was inserted into a 15-mL falcon tube. Afterward, 5 mL of solvent containing ultra-pure water and methanol (1/1, v/v) was added to the tube, and the sample was sonicated for 30 min at 40C and then centrifuged at 6080 x g. The supernatant was filtered through a 0.22-m nylon filter. Consequently, 1 mL of filtered solution was transferred into a dark vial and analyzed by UHPLC-Q-Orbitrap-HRMS as described previously. Procedure blanks consisting of water and methanol (1/1, v/v) were injected after each analyzed sample for background interference removal. Two standard calibrated solutions, Pierce LTQ Velos ESI Positive Ion and Pierce LTQ Velos ESI Negative Ion, were used at the beginning, at the end of each run and after each batch of fifteen analyzed samples.