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MB Sample ID: SA211305
Local Sample ID: | Tsu-0_W-N+_P3ID34_b |
Subject ID: | SU002287 |
Subject Type: | Plant |
Subject Species: | Arabidopsis thaliana |
Taxonomy ID: | 3702 |
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Sample Preparation:
Sampleprep ID: | SP002293 |
Sampleprep Summary: | All steps were performed in 2 ml Safelock Eppendorf tubes. The ground frozen samples (25 mg) were resuspended in 1 ml of frozen (-20°C) Water:Acetonitrile:Isopropanol (2:3:3) containing Ribitol at 4 µg/ml and extracted for 10 min at 4°C with shaking at 1400 rpm in an Eppendorf Thermomixer. Insoluble material was removed by centrifugation at 20000g for 5 min. 66 µl were collected and dried overnight at 35 °C in a Speed-Vac . Fiehn et al (the Plant Journal (2008) 53, 691-704). |
Processing Storage Conditions: | -20℃ |
Extract Storage: | -80℃ |