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MB Sample ID: SA211341
| Local Sample ID: | ES250414-PDA1 |
| Subject ID: | SU002288 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 (WT); LSL-KrasG12D/+ - p48Cre/+; LSL-KrasG12/D+; LSL-Tpr53R172H/+; Pdx-1-Cre |
| Age Or Age Range: | 1.5 to 9 months |
| Animal Light Cycle: | 12h light/dark cycles (7.00 AM lights on) |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
| Animal Inclusion Criteria: | Healthy animails |
| Species Group: | C57BL/6 (WT); LSL-KrasG12D/+ - p48Cre/+; LSL-KrasG12/D+; LSL-Tpr53R172H/+; Pdx-1-Cre |
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Sample Preparation:
| Sampleprep ID: | SP002294 |
| Sampleprep Summary: | Tissues (weights in the 60-120 mg range) were homogenized (ca. 200mg/mL) in 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 1 mM EDTA (ethylenediaminetetraacetic acid), 0.7% sodium deoxy- cholate, 1% Nonidet P-40, 0.5 M lithium chloride, pH 7.6, using a Precellys 24 homogeniser (Stretton Scientific, Stretton, UK) and then extracted in methanol:chloroform:water. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Biphasic method (methanol/ chloroform/ water) |
| Extract Storage: | -80℃ |
