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MB Sample ID: SA211464
Local Sample ID: | Maryam_2nd-set_Urine_DC_Vaccine_D9_D16 |
Subject ID: | SU002294 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Gender: | Not applicable |
Animal Animal Supplier: | Jackson Labs (Bar Harbor, ME) |
Animal Housing: | Housed in a temperature of 22 oC |
Animal Light Cycle: | 12-hour light/12-hour dark |
Animal Water: | free access to food and water (3-5 animals per cage). |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002300 |
Sampleprep Summary: | No filtration was performed for urine samples. The urine samples were centrifuged at 4 oC, with 13.2 K rpm speed before mixing it with an internal reference and phosphate buffer system. |
Sampleprep Protocol Filename: | DC_Vaccine_treated_NMR_urine_Procedures.docx |
Processing Method: | None |
Processing Storage Conditions: | -80℃ |
Extraction Method: | None |
Extract Storage: | -80℃ |
Sample Resuspension: | In 500 microliter of 150 mM phosphate buffer (pH 7.2) with 2 mM EDTA, 0.5 mM DSS and 0.2% sodium azide for aqueous phase samples. |
Sample Spiking: | 0.5 mM DSS for urine samples |