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MB Sample ID: SA212970
Local Sample ID: | PL127_1259011 |
Subject ID: | SU002321 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 50 +/-11 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP002327 |
Sampleprep Summary: | Plasma metabolites were extracted from pre-aliquoted biospecimens (15µL) with 45µL of LCMS grade methanol (ThermoFisher) in a 96-well microplate (Eppendorf). Plates were heat sealed, vortexed for 5 min at 750 rpm, and centrifuged at 2000 × g for 10 minutes at room temperature. The supernatant (30µL) was transferred to a 96-well plate, leaving behind the precipitated protein. The supernatant was further diluted with 60µL of 100mM ammonium formate, pH3 (Fisher Scientific). For Hydrophilic Interaction Liquid Chromatography (HILIC) positive ion analysis, 15µL of the supernatant and ammonium formate mix were diluted with 195µL of 1:3:8:144 water (GenPure ultrapure water system, Thermofisher): LCMS grade methanol (ThermoFisher): 100mM ammonium formate, pH3 (Fisher Scientific): LCMS grade acetonitrile (ThermoFisher). For C18 analysis, 15µL of the supernatant and ammonium formate mix were diluted with 90µL water (GenPure ultrapure water system, ThermoFisher) for positive ion mode. Each sample solution was transferred to 384-well microplate (Eppendorf) for LCMS analysis. |