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MB Sample ID: SA214109
| Local Sample ID: | 4_GAT_4-neg |
| Subject ID: | SU002326 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Age Or Age Range: | 10 day old seedlings |
| Species Group: | Roots |
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Sample Preparation:
| Sampleprep ID: | SP002332 |
| Sampleprep Summary: | Frozen Arabidopsis root tissue was homogenized using a tissue lyser, and extraction was carried out using 1 mL of 4:1 methanol: water (v/v) with incubation in an ultra-sonication bath for 30 min followed by shaking for 30 min at 4°C. The mixture was then centrifuged at 21,000 x g for 10 min at 4°C; supernatant was transferred into fresh tubes and evaporated to dryness using a speedvac centrifuge at ambient temperature. Dried residue was re-suspended in 200 µL of 1:1 methanol: water (v/v), filtered using 0.2 µm PTFE micro centrifuge filters and transferred to glass vials for HILIC-HRMS runs. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | 4:1 Methanol:Water |
