Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA214425

Local Sample ID:	Carrier_Positive_P-21
Subject ID:	SU002330
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation
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MS

Sample Preparation:

Sampleprep ID:	SP002336
Sampleprep Summary:	20 μL plasma, was mixed with 30 μL internal standard working solution, and extracted with 200 μL methanol for 10 min, and precipitated protein at -20℃ for 1h. After centrifugation at 14000xg for 10 min at 4 ℃, the supernatant was transferred into a new tube, dried under nitrogen, and resuspended in 50 μL acetonitrile/water (75/25, v/v). 5 μL of each sample was combined to make a pooled quality control (QC) sample and ran every ten samples in the long sequence to monitor retention time and signal intensity drift. The remaining samples (30 μL) were transferred into injection vials for metabolomic analysis.

Sampleprep ID:

SP002336

Sampleprep Summary:

20 μL plasma, was mixed with 30 μL internal standard working solution, and extracted with 200 μL methanol for 10 min, and precipitated protein at -20℃ for 1h. After centrifugation at 14000xg for 10 min at 4 ℃, the supernatant was transferred into a new tube, dried under nitrogen, and resuspended in 50 μL acetonitrile/water (75/25, v/v). 5 μL of each sample was combined to make a pooled quality control (QC) sample and ran every ten samples in the long sequence to monitor retention time and signal intensity drift. The remaining samples (30 μL) were transferred into injection vials for metabolomic analysis.