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MB Sample ID: SA220309
Local Sample ID: | xeno_B_EL_A_25b_1_2 |
Subject ID: | SU002379 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | CBA nude (N:NIH(S)II-nu/nu) |
Age Or Age Range: | 6 to 7-weeks old |
Gender: | Female |
Animal Animal Supplier: | i3S Animal Facility (Porto, Portugal) |
Animal Housing: | ICBAS-UP Rodent Animal House Facility (Porto, Portugal) |
Animal Light Cycle: | 12h light/dark cycles (7.00 AM lights on) |
Animal Feed: | ad libitum |
Animal Water: | ad libitum |
Animal Inclusion Criteria: | Healthy animails |
Species Group: | CBA nude (N:NIH(S)II-nu/nu) |
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Sample Preparation:
Sampleprep ID: | SP002385 |
Sampleprep Summary: | The frontal cortex of each mice brain was mechanically grounded in liquid nitrogen and samples were extracted using the biphasic methanol/ chloroform/ water (2:2:1) method. Lipophilic phases were recovered and dried. Previously to NMR acquisition, lipophilic extracts were suspended in 650 µL of CDCl3, containing 0.03% tetramethylsilane (TMS). Samples were then homogenized and transferred into 5mm NMR tubes. |
Processing Storage Conditions: | -80℃ |
Extraction Method: | Biphasic method (methanol/ chloroform/ water) |
Extract Storage: | -80℃ |