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MB Sample ID: SA220372
| Local Sample ID: | xeno_L_EL_B_19b_1_2 |
| Subject ID: | SU002381 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | CBA nude (N:NIH(S)II-nu/nu) |
| Age Or Age Range: | 6 to 7-weeks old |
| Gender: | Female |
| Animal Animal Supplier: | i3S Animal Facility (Porto, Portugal) |
| Animal Housing: | ICBAS-UP Rodent Animal House Facility (Porto, Portugal) |
| Animal Light Cycle: | 12h light/dark cycles (7.00 AM lights on) |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
| Animal Inclusion Criteria: | Healthy animails |
| Species Group: | CBA nude (N:NIH(S)II-nu/nu) |
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Sample Preparation:
| Sampleprep ID: | SP002387 |
| Sampleprep Summary: | The half of liver (comprising median and left lobes) from each mouse was mechanically grounded in liquid nitrogen and samples were extracted using the biphasic methanol/ chloroform/ water (2:2:1) method. Lipophilic phases were recovered and dried. Previously to NMR acquisition, lipophilic extracts were suspended in 650 µL of CDCl3, containing 0.03% tetramethylsilane (TMS). Samples were then homogenized and transferred into 5mm NMR tubes. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Biphasic method (methanol/ chloroform/ water) |
| Extract Storage: | -80℃ |
