Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA220375

Local Sample ID:	P39_V6
Subject ID:	SU002382
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002388
Sampleprep Summary:	Serum samples were prepared using the Agilent Bravo metabolomics platform. This automated platform was programmed to pipette 100 µL of serum into a 96-well plate from a 96-well plate that contained 200 µL of serum. Following this, 450 μL of a 1:1 mixture of methanol:ethanol was added to the plate to precipitate proteins. The supernatant was then applied to a Captiva EMR-lipid 96-well plate and left to stand for 5 min and a vacuum was applied to the plate (2-5 Hg) to initiate flow. Eluents were collected into 1 mL 96-well plates and subsequently dried under a stream of nitrogen. Prior to analysis, samples were reconstituted with 100 µL of 10 % methanol and were agitated on a plate shaker (MTS 2/4m IKA) at 600 rpm for 10 min.

Sampleprep ID:

SP002388

Sampleprep Summary:

Serum samples were prepared using the Agilent Bravo metabolomics platform. This automated platform was programmed to pipette 100 µL of serum into a 96-well plate from a 96-well plate that contained 200 µL of serum. Following this, 450 μL of a 1:1 mixture of methanol:ethanol was added to the plate to precipitate proteins. The supernatant was then applied to a Captiva EMR-lipid 96-well plate and left to stand for 5 min and a vacuum was applied to the plate (2-5 Hg) to initiate flow. Eluents were collected into 1 mL 96-well plates and subsequently dried under a stream of nitrogen. Prior to analysis, samples were reconstituted with 100 µL of 10 % methanol and were agitated on a plate shaker (MTS 2/4m IKA) at 600 rpm for 10 min.