Return to study ST002322 main page
MB Sample ID: SA227400
Local Sample ID: | KO_LPS_24hr_3 |
Subject ID: | SU002408 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | SCAP fl/fl CD19-Cre (KO) and SCAP+/+ CD19-Cre (WT) |
Cell Strain Details: | Splenic B cell |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002414 |
Sampleprep Summary: | Sample preparation: Proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phases (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis. |