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MB Sample ID: SA232159
Local Sample ID: | S25 |
Subject ID: | SU002423 |
Subject Type: | Invertebrate |
Subject Species: | Drosophila melanogaster |
Taxonomy ID: | 7227 |
Age Or Age Range: | 15 day old flies |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP002429 |
Sampleprep Summary: | Lipids were extracted using chloroform and methanol (1). Prior to lipid extraction, samples were spiked with lipid class-specific internal standards. After drying and resuspending in an appropriate MS acquisition mixture, lipid extracts were infused directly in QExactive mass spectrometer (Thermo Fisher Scientific) with TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Sample preparations and analysis were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011). |