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MB Sample ID: SA235724
| Local Sample ID: | RS_M6-1_1 |
| Subject ID: | SU002439 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 6-8 weeks |
| Gender: | Female |
| Animal Animal Supplier: | The Jackson Laboratory, Bar Harbor, ME |
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Sample Preparation:
| Sampleprep ID: | SP002445 |
| Sampleprep Summary: | The BALF samples were allowed to thaw at 4°C overnight, and then split into technical triplicates of 200 μL that were transferred and sealed into sterilized 2mL GC headspace vials with Supelco® PTFE/silicone septum magnetic screw caps (Sigma-Aldrich®, St. Louis, MO). Samples were randomized for analysis. Volatile metabolites sampling was performed by solid phase microextraction (SPME) using a Gerstel® MPS Robotic Pro MultiPurpose autosampler directed by Maestro® software (Gerstel®, Inc., Linthicum, MD). Sample extraction and injection parameters are provided in Table S3 (see Autosampler Method). Volatile metabolite analysis was performed by two-dimensional gas chromatography−time-of-flight mass spectrometry (GC×GC–TOFMS) using a LECO® Pegasus® 4D and Agilent® 7890B GC (LECO® Corp., St. Joseph, MI). Chromatographic, mass spectrometric, and peak detection parameters are provided in Table S3 (see GC×GC Method and Mass Spectrometry Method). An external alkane standards mixture (C8 – C20; Sigma-Aldrich®) was sampled multiple times for calculating retention indices (RI). The injection, chromatographic, and mass spectrometric methods for analyzing the alkane standards were the same as for the samples. |
| Extraction Method: | Solid-phase microextraction (SPME) |
