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MB Sample ID: SA237415
| Local Sample ID: | WT-LPS-3 |
| Subject ID: | SU002467 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
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Sample Preparation:
| Sampleprep ID: | SP002473 |
| Sampleprep Summary: | Macrophages were lysed, and polar metabolites were extracted using methanol and H2O (80:20; HPLC Grade; Sigma-Aldrich). Briefly, After treatment, immediately aspirate medium at room temperature. Immediately place the plate on dry ice, and add 1 mL 80% methanol/water (both HPLC grade) (pre-cooled in -80oC for at least 1hr).Remove the plate from -80oC freezer and put it on dry ice, scrape cells into extraction solvent. Transfer the whole cell extract to a new Eppendorf tube placed on ice. Centrifuge at 20 000 rcf for 10 min, 4oC.Transfer the supernatant into two tubes and dry with a speed vacuum at room temperature. The dried metabolites were stored at -80 freezer before analysis. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | Described in summary |
