Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA239029

Local Sample ID:	m-41RL
Subject ID:	SU002485
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation
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MS

Sample Preparation:

Sampleprep ID:	SP002491
Sampleprep Summary:	Mouse liver sections were weighed into Eppendorf tubes. The liver tissue was then mashed with a spatula prior to extraction. The samples were extracted using a liquid liquid partition with water (250 µL), methanol (300 µL), and MTBE (1 mL). Avanti’s deuterated lipid mix, Equisplash, was used as an internal standard. This was spiked into the methanol at 1.5 µg/mL and used for extraction. The extracts were centrifuged at 20,000 rcf for 10 min. The top layer was removed, dried down, and reconstituted in 1 mL of IPA for analysis.

Sampleprep ID:

SP002491

Sampleprep Summary:

Mouse liver sections were weighed into Eppendorf tubes. The liver tissue was then mashed with a spatula prior to extraction. The samples were extracted using a liquid liquid partition with water (250 µL), methanol (300 µL), and MTBE (1 mL). Avanti’s deuterated lipid mix, Equisplash, was used as an internal standard. This was spiked into the methanol at 1.5 µg/mL and used for extraction. The extracts were centrifuged at 20,000 rcf for 10 min. The top layer was removed, dried down, and reconstituted in 1 mL of IPA for analysis.