Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA239105

Local Sample ID:	CTL_04_LIP329
Subject ID:	SU002489
Subject Type:	Cultured cells
Subject Species:	Homo sapiens

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Sample Preparation:

Sampleprep ID:	SP002495
Sampleprep Summary:	Lipid extraction. Lipids were extracted from ASC cells according to a modified Folch method. Cell pellets were resuspended in 100µl methanol and supplemented with deuterated internal standards. Lipids were extracted with 1.5 mL chloroform and 650 µL methanol, sonicated for 15 min. Phase separation was triggered by addition of 450 µL of ammonium carbonate (250 mM). Lower organic phase was dried and resuspended in 200 µL of liquid chromatography – mass spectrometry (LC-MS) solvent.

Sampleprep ID:

SP002495

Sampleprep Summary:

Lipid extraction. Lipids were extracted from ASC cells according to a modified Folch method. Cell pellets were resuspended in 100µl methanol and supplemented with deuterated internal standards. Lipids were extracted with 1.5 mL chloroform and 650 µL methanol, sonicated for 15 min. Phase separation was triggered by addition of 450 µL of ammonium carbonate (250 mM). Lower organic phase was dried and resuspended in 200 µL of liquid chromatography – mass spectrometry (LC-MS) solvent.