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MB Sample ID: SA240888
| Local Sample ID: | C18-6-neg |
| Subject ID: | SU002495 |
| Subject Type: | Plant |
| Subject Species: | Brassica napus |
| Taxonomy ID: | 3708 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002501 |
| Sampleprep Summary: | A total of 27 samples from the three treatments (PC, Si0.5 and Si1.0) were used for metabolomic analyses at 7-, 14- and 21 dpi. Hi-Q seedlings were grown in the soil-mix as previously described with/without Si amendment, inoculated with P. brassicae, and subsequently, washed thoroughly, flash-frozen in liquid nitrogen and stored at -80°C until analysis. Each biological replicate was comprised of 5 individual plants per treatment, and three biological replicates were generated from three independent experiments. A total of 27 samples were generated for transcriptomics and metabolomics analyses [3 treatments (PC, Si0.5 and Si1.0) x 3 time-points (7-, 14- and 21 dpi) x 3 biological replicates]. |
| Sampleprep Protocol Filename: | Sample Prep protocol |
| Processing Storage Conditions: | -20℃ |
| Extraction Method: | 50% ACN |
| Extract Cleanup: | HLB SPE |
| Extract Storage: | -20℃ |
| Sample Resuspension: | Primary metabolites - 90% ACN, glucosinolates - 5% ACN |
| Sample Spiking: | Primary metabolites - Labeled amino acid mix; Glucosinolates - labeled CK mix |
