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MB Sample ID: SA242932
Local Sample ID: | OilPalm_Drought_Control_R3_POS |
Subject ID: | SU002519 |
Subject Type: | Plant |
Subject Species: | Elaeis guineensis Jacq. |
Taxonomy ID: | NCBI:txid51953 |
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Sample Preparation:
Sampleprep ID: | SP002525 |
Sampleprep Summary: | Leaf samples with approximately 50 mg were collected for the metabolomics analysis; four replicates per plant. After harvesting, samples were immediately frozen in liquid nitrogen and stored at − 80 °C until metabolites extraction and analysis. Each sample was ground in a ball mill (Biospec Products, USA) before solvent extraction. Metabolites were extracted using an adapted protocol from The Max Planck Institute, called All-in-One, which provides a polar fraction for secondary metabolite analysis, a nonpolar fraction for lipidomics, and a protein pellet for proteomics; all obtained from the same plant sample. Each ground sample was added to a microtube and mixed with 1 mL of a methanol and methyl-tert-butyl-ether (1:3) solution at − 20°C. After homogenization, they were incubated at 4 °C for 10 min. Each microtube was ultrasonicated in an ice bath for another 10 min. Then, 500 μL of a methanol and water (1:3) solution was added to the microtube before centrifugation (12,000 rpm at 4 °C for 5 min). Three phases were separate: an upper non-polar (green), a lower polar (brown), and a remaining protein pellet. Samples were transferred to fresh microtubes and vacuum-dried in a speed vac (Centrivap, Labconco, Kansas City, MO, USA) overnight at room temperature (~ 22 °C). |