Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA244202

Local Sample ID:	SC_20201109_HILICp_FMS_CSF_D09_C_1_2
Subject ID:	SU002531
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

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Sample Preparation:

Sampleprep ID:	SP002537
Sampleprep Summary:	CSF samples collected were flash frozen and stored at -80°C until analyzed via Liquid ChromatographyHigh Resolution Mass Spectrometry (LC-HRMS and LC-HRMS/MS)-based metabolomics in the Vanderbilt Center for Innovative Technology (CIT) using previously described methods (cite). Briefly, equal volumes (100 µL) of previously frozen CSF was diluted with 100 µL ice-cold lysis buffer (1:1:2, Acetonitrile:MeOH:Ammonium Bicarbonate 0.1M, pH 8.0, LC-MS grade). Addition of isotopically labeled phenylalanine-D8 and biotin-D2 were added to individual samples prior to protein precipitation by addition of 800 µL of ice-cold methanol. Following overnight incubation at -80°C, precipitated proteins were pelleted by centrifugation at 10,000 rpm for 10 min and metabolite extracts were dried down in vacuo and stored at -80°C until reconstitution prior to MS analysis.

Sampleprep ID:

SP002537

Sampleprep Summary:

CSF samples collected were flash frozen and stored at -80°C until analyzed via Liquid ChromatographyHigh Resolution Mass Spectrometry (LC-HRMS and LC-HRMS/MS)-based metabolomics in the Vanderbilt Center for Innovative Technology (CIT) using previously described methods (cite). Briefly, equal volumes (100 µL) of previously frozen CSF was diluted with 100 µL ice-cold lysis buffer (1:1:2, Acetonitrile:MeOH:Ammonium Bicarbonate 0.1M, pH 8.0, LC-MS grade). Addition of isotopically labeled phenylalanine-D8 and biotin-D2 were added to individual samples prior to protein precipitation by addition of 800 µL of ice-cold methanol. Following overnight incubation at -80°C, precipitated proteins were pelleted by centrifugation at 10,000 rpm for 10 min and metabolite extracts were dried down in vacuo and stored at -80°C until reconstitution prior to MS analysis.