Return to study ST002481 main page
MB Sample ID: SA248227
Local Sample ID: | 1_UroA_10_2 |
Subject ID: | SU002571 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002577 |
Sampleprep Summary: | 40 µg microsomes isolated from Hepa 1 cells treated with 5 nM TCDD for 24 h, or lung microsomes (100 µg) from mice on the semi-purified diet, were incubated with various compounds and incubation times as indicated. The reactions were carried out at 37°C in an incubator in the presence of NADPH regeneration system and terminated by addition of 800 µL ice cold 100% methanol (v/v) containing 1 µM chlorpropamide as internal standard. Then mixtures were kept in -20°C for 30 mins followed by centrifugation at 12,000 × g for 20 min at 4°C. The supernatants were dried in a under vacuum and reconstituted in 60 μL 50% methanol (v/v). After centrifugation, supernatants were transferred to autosampler vials for LC-MS/MS analysis. |