Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA248262

Local Sample ID:	3_ICZ_120_2
Subject ID:	SU002571
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation:

Sampleprep ID:	SP002577
Sampleprep Summary:	40 µg microsomes isolated from Hepa 1 cells treated with 5 nM TCDD for 24 h, or lung microsomes (100 µg) from mice on the semi-purified diet, were incubated with various compounds and incubation times as indicated. The reactions were carried out at 37°C in an incubator in the presence of NADPH regeneration system and terminated by addition of 800 µL ice cold 100% methanol (v/v) containing 1 µM chlorpropamide as internal standard. Then mixtures were kept in -20°C for 30 mins followed by centrifugation at 12,000 × g for 20 min at 4°C. The supernatants were dried in a under vacuum and reconstituted in 60 μL 50% methanol (v/v). After centrifugation, supernatants were transferred to autosampler vials for LC-MS/MS analysis.

Sampleprep ID:

SP002577

Sampleprep Summary:

40 µg microsomes isolated from Hepa 1 cells treated with 5 nM TCDD for 24 h, or lung microsomes (100 µg) from mice on the semi-purified diet, were incubated with various compounds and incubation times as indicated. The reactions were carried out at 37°C in an incubator in the presence of NADPH regeneration system and terminated by addition of 800 µL ice cold 100% methanol (v/v) containing 1 µM chlorpropamide as internal standard. Then mixtures were kept in -20°C for 30 mins followed by centrifugation at 12,000 × g for 20 min at 4°C. The supernatants were dried in a under vacuum and reconstituted in 60 μL 50% methanol (v/v). After centrifugation, supernatants were transferred to autosampler vials for LC-MS/MS analysis.