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MB Sample ID: SA253889
Local Sample ID: | BK5 |
Subject ID: | SU002618 |
Subject Type: | Bacteria |
Subject Species: | Eggerthella lenta |
Taxonomy ID: | 84112 |
Genotype Strain: | DSM 2243 |
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Sample Preparation:
Sampleprep ID: | SP002624 |
Sampleprep Summary: | Bacterial culture supernatant and sterile media, used in culture, were thawed on wet ice. Once thawed, samples were homogenized by inversion five times. Extracellular culture supernatant samples were prepared as follows: 20 μL of culture supernatant were extracted using 80 μL of a chilled extraction solvent at −20°C (1:1 acetonitrile:methanol, 5% water containing stable isotope-labeled internal standards). Samples were homogenized via pipette action, incubated for 1 hour at −20°C, centrifuged at 4°C at 6000 rcf for 5 min. The supernatant was transferred to a new plate and immediately sealed and kept at 4°C prior to prompt analysis via LC-MS/MS. |