Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA254456

Local Sample ID:	BUT5
Subject ID:	SU002621
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP002627
Sampleprep Summary:	Serum samples were thawed on ice, followed by metabolite extraction of the non-polar (lipids) metabolome. Extraction solvent was prepared by adding 725 μL of the isotopically labeled lipid standard mixture to 43.5 mL 2-propanol (1:60 ratio) and was kept on ice. This cold extraction solvent was added to serum samples in a 3:1 ratio (solvent: serum) for protein precipitation followed by vortex mixing for 15 seconds. Samples were centrifuged at 13,000 rpm for 7 minutes and the resulting supernatant was transferred to LC vials. The supernatant was stored at -80 °C until UHPLC-MS analysis, which was performed within one week of preparation. A blank sample, prepared with LC-MS grade water, underwent the same sample preparation process as the serum samples and was analyzed with the rest of the samples. Pooled quality control (QC) samples were prepared by combining 5-10 μL aliquots of the supernatant of each serum sample. This pooled QC sample was analyzed after every 10 LC-MS runs to monitor instrument stability through the course of the experiment. Samples were randomized for sample preparation and were run in a randomized order on consecutive days.

Sampleprep ID:

SP002627

Sampleprep Summary:

Serum samples were thawed on ice, followed by metabolite extraction of the non-polar (lipids) metabolome. Extraction solvent was prepared by adding 725 μL of the isotopically labeled lipid standard mixture to 43.5 mL 2-propanol (1:60 ratio) and was kept on ice. This cold extraction solvent was added to serum samples in a 3:1 ratio (solvent: serum) for protein precipitation followed by vortex mixing for 15 seconds. Samples were centrifuged at 13,000 rpm for 7 minutes and the resulting supernatant was transferred to LC vials. The supernatant was stored at -80 °C until UHPLC-MS analysis, which was performed within one week of preparation. A blank sample, prepared with LC-MS grade water, underwent the same sample preparation process as the serum samples and was analyzed with the rest of the samples. Pooled quality control (QC) samples were prepared by combining 5-10 μL aliquots of the supernatant of each serum sample. This pooled QC sample was analyzed after every 10 LC-MS runs to monitor instrument stability through the course of the experiment. Samples were randomized for sample preparation and were run in a randomized order on consecutive days.