Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA255061

Local Sample ID:	Purslane_SP4_Adult_Leaf_Lipidic_Negative
Subject ID:	SU002637
Subject Type:	Plant
Subject Species:	Portulaca oleracea
Taxonomy ID:	46147

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Sample Preparation:

Sampleprep ID:	SP002643
Sampleprep Summary:	For the metabolomics study, the following substances were acquired from Sigma Aldrich (Merck, USA): methanol UHPLC grade, acetonitrile LC-MS grade, methyl-tert-butyl-ether, formic acid LC-MS grade, and sodium hydroxide ACS grade. Water was obtained using a Milli-Q system (Millipore, USA). Metabolites were extracted using a well-established protocol, which provides polar and lipidic fractions from the same samples. In this protocol, we first ground the plant material (roots or leaves) in a ball mill (Biospec Products, USA), then added to a microtube containing 1 mL from a solution (1:3) of methanol and methyl-tert-butyl-ether at -20°C. Samples were incubated for 10 min at 4.0 °C, then ultrasonicated for another 10 min in an ice bath. A solution (1:3) of methanol and water was added to each microtube and then submitted to centrifugation (12,000 rpm at 4.0°C for 5 min). The polar (upper) and non-polar (lower) fractions were collected and vacuum-dried in a speed vac system overnight (Centrivap, Labconco, Kansas City, MO, USA). Four microliters of the extract were resuspended in 850 μL of the methanol and water (1:3) solvent mixture and then analyzed by UHPLC-MS.

Sampleprep ID:

SP002643

Sampleprep Summary:

For the metabolomics study, the following substances were acquired from Sigma Aldrich (Merck, USA): methanol UHPLC grade, acetonitrile LC-MS grade, methyl-tert-butyl-ether, formic acid LC-MS grade, and sodium hydroxide ACS grade. Water was obtained using a Milli-Q system (Millipore, USA). Metabolites were extracted using a well-established protocol, which provides polar and lipidic fractions from the same samples. In this protocol, we first ground the plant material (roots or leaves) in a ball mill (Biospec Products, USA), then added to a microtube containing 1 mL from a solution (1:3) of methanol and methyl-tert-butyl-ether at -20°C. Samples were incubated for 10 min at 4.0 °C, then ultrasonicated for another 10 min in an ice bath. A solution (1:3) of methanol and water was added to each microtube and then submitted to centrifugation (12,000 rpm at 4.0°C for 5 min). The polar (upper) and non-polar (lower) fractions were collected and vacuum-dried in a speed vac system overnight (Centrivap, Labconco, Kansas City, MO, USA). Four microliters of the extract were resuspended in 850 μL of the methanol and water (1:3) solvent mixture and then analyzed by UHPLC-MS.