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MB Sample ID: SA257696
| Local Sample ID: | wt1_ep40 |
| Subject ID: | SU002662 |
| Subject Type: | Fish |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Genotype Strain: | WT/PMM2 mutant sa10150 |
| Age Or Age Range: | 6 dpf |
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Sample Preparation:
| Sampleprep ID: | SP002668 |
| Sampleprep Summary: | 10 embryos per sample were homogenized by sonication in extraction buffer (80% MeOH, IS). The metabolites were then precipitated at -80 °C overnight, after which the samples were centrifuged at 15,000 rpm, 20 min, 4 °C. Next, the supernatant was transferred to a fresh Eppendorf tube. The samples were centrifuged again at 15,000 rpm, 20 min, 4 °C to remove any leftover impurities and transferred to a fresh MS vial. Finally, the samples were analyzed by LC/MS |
| Processing Storage Conditions: | -80? |
| Extraction Method: | 80 % MeOH with Internal standards (e.g. d27 myristic acid) |
