Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA258347

Local Sample ID:	QC01-NEG
Subject ID:	SU002671
Subject Type:	Plant
Subject Species:	Pitcairnia flammea

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Sample Preparation:

Sampleprep ID:	SP002677
Sampleprep Summary:	Lipid extraction was based on Hummel et al. Method. 10 mL of solvent mixture were prepared in 3 different proportions - 1:2, 1:3 or 1:4 (v/v) -, pre-cooled at 20 ºC methanol (MeOH) and methyl tert-butyl ether (MTBE). In 2 mL tubes, 50 mg of macerated sample were added and 1 mL of the solvent mixture. The samples were incubated for 5 min under agitation at 500 rpm at 4 ºC, followed by an ultrasonication in ice-cold bath in different times (10, 20, 30 minutes). Each condition was performed in technical triplicate, totalizing 27 samples. After adding 500 µL mixture of water type I:MeOH (3:1 v/v), the samples were vortexed and centrifuged for 5 min at 4 ºC, 10000 rpm. The three phases were separated and dried in a vacuum concentrator, at ambient temperature under vacuum - alcoholic mode, and stored at -80 ºC until the chromatographic analysis.

Sampleprep ID:

SP002677

Sampleprep Summary:

Lipid extraction was based on Hummel et al. Method. 10 mL of solvent mixture were prepared in 3 different proportions - 1:2, 1:3 or 1:4 (v/v) -, pre-cooled at 20 ºC methanol (MeOH) and methyl tert-butyl ether (MTBE). In 2 mL tubes, 50 mg of macerated sample were added and 1 mL of the solvent mixture. The samples were incubated for 5 min under agitation at 500 rpm at 4 ºC, followed by an ultrasonication in ice-cold bath in different times (10, 20, 30 minutes). Each condition was performed in technical triplicate, totalizing 27 samples. After adding 500 µL mixture of water type I:MeOH (3:1 v/v), the samples were vortexed and centrifuged for 5 min at 4 ºC, 10000 rpm. The three phases were separated and dried in a vacuum concentrator, at ambient temperature under vacuum - alcoholic mode, and stored at -80 ºC until the chromatographic analysis.