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MB Sample ID: SA258352
| Local Sample ID: | ap1calAP1GR_cytokinins_3 |
| Subject ID: | SU002672 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Genotype Strain: | ap1 cal AP1-GR and drmy1 ap1 cal AP1-GR |
| Age Or Age Range: | Bolting (40-50 days after germination) |
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Sample Preparation:
| Sampleprep ID: | SP002678 |
| Sampleprep Summary: | Samples were ground in liquid nitrogen and twice extracted in methanol : water : formic acid (15:4:1). 200 pg of BAP per sample was added as an internal control. Extracts were centrifuged at 14,650 rpm in -4°C for 30 min, and supernatant was evaporated of methanol and reconstituted in 1% (v/v) acetic acid. Samples were passed through an Oasis MCX SPE column (Waters 186000252), washed with 1% acetic acid, washed with methanol, and eluted with 0.35 M ammonia in 70% methanol. Eluents were evaporated to complete dryness, reconstituted in 5% acetonitrile, and sent for LC-MS. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Modified Bieleski buffer, methanol : water : formic acid (15:4:1) |
| Extract Enrichment: | An Oasis MCX SPE column (Waters 186000252) was used to enrich for cytokinins |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 5% acetonitrile |
| Sample Spiking: | 200 pg of BAP per sample was added as an internal control |
