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MB Sample ID: SA273827
| Local Sample ID: | 13_1 |
| Subject ID: | SU002827 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Age Or Age Range: | 12 weeks |
| Gender: | Male |
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Sample Preparation:
| Sampleprep ID: | SP002833 |
| Sampleprep Summary: | Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, lung samples were removed from storage at -80 degrees C and thawed on ice. The 20-30mg of each lung tissue samples was then mixed with ice cold LC-MS grade acetonitrile: water mixture (Sigma-Aldrich; 2:1 ration; 15uL per mg tissue) which contains 2.5uL per 100uL of internal standard solution with eight stable isotopic chemicals selected to cover a range of chemical properties. Samples are then sonicated on ice to ensure metabolite extraction as previously described (Chandler et al (2016) AJ Physiology; Hu et al. (2019) AJ Pathology). Following sonication, tissue lysates are then equilibrated for 30 mins on ice, upon which precipitated proteins are removed by centrifuge (16.1xg at 4C for 10 mins). The resulting supernatant containing metabolite extract (100uL) is removed, added to a low volume autosampler vial and maintained at 4C until analysis (<22h). |
| Sampleprep Protocol Filename: | EmoryUniversity_HRM_SamplePreparation_tissue_062016_v1.pdf |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
