Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA288559

Local Sample ID:	DM_1
Subject ID:	SU002840
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Female
Cell Biosource Or Supplier:	American Tissue Culture Collection (ATCC)
Cell Strain Details:	HEK-293T
Cell Passage Number:	2

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MS

Sample Preparation:

Sampleprep ID:	SP002846
Sampleprep Summary:	Approximately 10 million cells per sample were washed with PBS at room tem-perature and harvested with a mixture of ice-cold methanol:water (80:20). Cell lysis was carried out by four freeze-and-thaw cycles with liquid nitrogen and dry-ice with vortexing between cycles. Supernatant was then dried on SpeedVac concentrator and samples stored at -80ºC. Dried extracts were resuspended in a mixture of Acetonitrile:Water (25:75).

Sampleprep ID:

SP002846

Sampleprep Summary:

Approximately 10 million cells per sample were washed with PBS at room tem-perature and harvested with a mixture of ice-cold methanol:water (80:20). Cell lysis was carried out by four freeze-and-thaw cycles with liquid nitrogen and dry-ice with vortexing between cycles. Supernatant was then dried on SpeedVac concentrator and samples stored at -80ºC. Dried extracts were resuspended in a mixture of Acetonitrile:Water (25:75).