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MB Sample ID: SA288567
Local Sample ID: | QC_1_neg |
Subject ID: | SU002840 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Cell Biosource Or Supplier: | American Tissue Culture Collection (ATCC) |
Cell Strain Details: | HEK-293T |
Cell Passage Number: | 2 |
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Sample Preparation:
Sampleprep ID: | SP002846 |
Sampleprep Summary: | Approximately 10 million cells per sample were washed with PBS at room tem-perature and harvested with a mixture of ice-cold methanol:water (80:20). Cell lysis was carried out by four freeze-and-thaw cycles with liquid nitrogen and dry-ice with vortexing between cycles. Supernatant was then dried on SpeedVac concentrator and samples stored at -80ÂșC. Dried extracts were resuspended in a mixture of Acetonitrile:Water (25:75). |