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MB Sample ID: SA288615
| Local Sample ID: | RS_4 |
| Subject ID: | SU002841 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
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Sample Preparation:
| Sampleprep ID: | SP002847 |
| Sampleprep Summary: | Metabolites were extracted from plasma were collected from 20 type2 diabetic patients, pre-and post-treatment with liraglutide (n=40 samples) (10). Briefly, 100 μL plasma sample were mixed with 900 μL of extraction solvent 50% acetonitrile (ACN) in methanol (MeOH). Meanwhile, QC samples were prepared with aliquots from all samples to check for system stability. The mixtures were mixed on thermomixer at 600 rpm at room temperature for one hour (Eppendorf, CITY, Germany). Afterward, the samples were centrifuged at 16000 rpm at 4ºC for 10 min. The supernatant was transferred into new Eppendrof tube, and then evaporated completely in a SpeedVac (Christ, Germany). The dried samples were reconstituted with100 μl of 50% mobile phase A: B (A: 0.1% Formic acid in dH2O, B: 0.1% Formic acid in 50% ACN: MeOH). |
| Sampleprep Protocol Filename: | Liraglutide_Metabolites_Extraction.docx |
