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MB Sample ID: SA288706
Local Sample ID: | RJ_33GJ1 |
Subject ID: | SU002843 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP002849 |
Sampleprep Summary: | Frozen LV tissue samples (~1-5mg) were weighed into Precellys tubes (Stretton Scientific Ltd., Derbyshire, UK), and an exact volume of extraction solution (50% methanol, 30% acetonitrile and 20% water) was added to obtain 40 mg specimen per ml of extraction solution, permitting comparisons between experimental conditions for the same metabolite. The samples were subsequently lysed after the addition of 3 ceramic beads using a Precellys 24 tissue homogenizer (Bertin Corp, Rockville, MD 20850, USA. 5500 r.p.m for 15 seconds × 2) and finally centrifuged (16,162 × g for 10 min at 4 °C). The supernatant was transferred into glass vials (Microsolv Technology Corp., Leland, NC 28451, USA) and stored at −80 °C until LC–MS analysis. |