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MB Sample ID: SA289348
| Local Sample ID: | MAR128-NEG |
| Subject ID: | SU002856 |
| Subject Type: | Plant |
| Subject Species: | Pitcairnia flammea |
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Sample Preparation:
| Sampleprep ID: | SP002862 |
| Sampleprep Summary: | Lipid extraction was based on Hummel et al. Method. 60 mL of solvent mixture was prepared with pre-cooled (-20ºC) methanol (MeOH, grade HPLC, LiChrosolv® Reag. Ph. Eur.) and methyl-tert-butyl-ether (MTBE, grade HPLC, purity 99,9%, Sigma-Aldrich) in proportion (1:3 v/v). In a 2 mL tube, 50 mg of macerated sample were added and 1 mL of the solvent mixture. The samples were incubated for 5 min under agitation at 500 rpm at 4 ºC (Microtube Shaking Incubator AccuTherm, Labnet International, Inc.), followed by an ultrasonication (Branson 5800 Ultrasonic Bath, Emerson, Danbury, USA) in ice-cold bath in 10 minutes. After adding 500 μL mixture of water type I:MeOH (3:1 v/v), the samples were vortexed and centrifuged for 5 min at 4 ºC, 10000 rpm (Hettich Zentrifugen Mikro 220R, Tuttlingen, DE). The three phases were separated and dried in a vacuum concentrator (Concentrator Plus, Eppendorf AG, Hamburg, DE), at ambient temperature under vacuum - alcoholic mode, and stored at -80 ºC until the chromatographic analysis. |
| Sampleprep Protocol Filename: | SamplePrep-BromeliadLipidomics.pdf |
