Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA305644

Local Sample ID:	WT_F_1F
Subject ID:	SU002933
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation:

Sampleprep ID:	SP002939
Sampleprep Summary:	For lipidomic metabolites extraction, 10 μL serum were mixed with 75 μL methanol and 250 μL Methyl tert-butyl ether (MTBE). After shaking for 6 minutes at 4 °C, 62 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further analysis. Polar metabolites were extracted from serum using the extraction buffer containing methanol: acetonitrile: H2O (40:40:20).

Sampleprep ID:

SP002939

Sampleprep Summary:

For lipidomic metabolites extraction, 10 μL serum were mixed with 75 μL methanol and 250 μL Methyl tert-butyl ether (MTBE). After shaking for 6 minutes at 4 °C, 62 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further analysis. Polar metabolites were extracted from serum using the extraction buffer containing methanol: acetonitrile: H2O (40:40:20).