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MB Sample ID: SA314223
Local Sample ID: | C74.1 |
Subject ID: | SU002986 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002992 |
Sampleprep Summary: | Briefly, 50 μL plasma spiked with 10 µL internal standard mixture and 10 µL BHT was blended into 380 µL methanol (75%). MTBE (1 mL) was added, and the mixture was vortexed for 30 min. Phase separation was induced by adding 250 µL H₂O. After incubation for 10 min at room temperature, the samples were centrifuged at 14 000 g for 10 min. The upper phase was collected and dried using a nitrogen evaporator. Ultra-performance liquid chromatography BEH C18 columns (1.7 μm, 100 × 2.1 mm i.d.) were used for chromatographic separations. |