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MB Sample ID: SA314496
Local Sample ID: | control-3-1 |
Subject ID: | SU002990 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP002996 |
Sampleprep Summary: | Cells were homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 °C and then centrifuged at 12,000 rpm for 10 min at 4 °C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The 2 supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. A total of 2 technical replicates were prepared for each sample. |