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MB Sample ID: SA323220
Local Sample ID: | 2A2-02-10480 |
Subject ID: | SU003089 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP003095 |
Sampleprep Summary: | Each collected serum sample (100 µL) was mixed with 300 µL of methanol (≥99.9 %, LC-MS CHROMASOLV) in Eppendorf tubes, followed by vortex and incubation at -20°C for 2 hours. After vortex and centrifugation at 14,000 rpm for 15 minutes, the supernatant was evaporated at 35-40°C using speed vacuum evaporation (EZ-2 Plus (GeneVac, Ipswich, UK). The extracted samples were resuspended in 200 µL of 0.1% formic acid in Deionized Water-LC-MS CHROMASOLV. Subsequently, the supernatant was filtered through a 0.45 µm pore size hydrophilic nylon syringe filter for LC-MS/MS analysis and collected in inserts within LC glass vials. All samples were placed in the autosampler at the temperature set at 4℃ to proceed with the analysis by Q-TOF MS. A pooled QC sample was created to ensure analysis reproducibility by mixing 10 µL from each sample. |