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MB Sample ID: SA323622

Local Sample ID:S_A24h_EA_22_1_2
Subject ID:SU003095
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:MDA-MB-231 cells
Gender:Not applicable
Cell Biosource Or Supplier:ATCC (Manassas, VA, USA); ATCC HTB-26
Cell Strain Details:Epithelial breast cancer cells; absence of estrogen and progesterone receptors, HER2 overexpression
Cell Passage Number:Inferior to 10
Cell Counts:5 M

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Sample Preparation:

Sampleprep ID:SP003101
Sampleprep Summary:The cellular polar extracts were extracted using a biphasic extraction method of methanol/chloroform/water. Basically, cell pellets were resuspended in 650 µL of 80% (v/v) methanol-miliQ water solution, transferred to microcentrifuge tubes with 150 mg of glass beads, and vortexed for 5 min. Subsequently, 260 µL of 100% chloroform and 260 µL of 100% chloroform plus 220 µL MiliQ water were added to samples, which were vortexed for 5 min between solvents addition. The samples were kept at − 20 °C for 10 min and centrifuged. The aqueous phase of the resulting extract was collected into a new tube, vacuum-dried and stored at − 80 °C until the NMR analysis. All samples and reagents were kept in ice during the extraction procedure. Before NMR analysis, the dry aqueous extracts were suspended in 650 µL of 100 mM sodium phosphate buffer (pH 7.4, in D2O containing 0.25% 3-(trimethylsilyl)propionic-2,2,3,3-D4 acid sodium salt (TSP-D4) for chemical shift referencing) and transferred into 5 mm NMR tubes.
Processing Storage Conditions:On ice
Extraction Method:Biphasic extraction method of methanol/chloroform/water
Extract Storage:-80℃
Sample Resuspension:Dry aqueous extracts were suspended in 650 µL of 100 mM sodium phosphate buffer (pH 7.4, in D2O containing 0.25% 3-(trimethylsilyl)propionic-2,2,3,3-D4 acid sodium salt (TSP-D4) for chemical shift referencing)
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