Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA326886

Local Sample ID:	EV_24h_C_neg_3
Subject ID:	SU003121
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090

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MS

Sample Preparation:

Sampleprep ID:	SP003127
Sampleprep Summary:	The adipocytes were homogenized in a TissueLyser II (Qiagen) (15 min 30 Hz) at 4 °C. 200 μL of the extracts were mixed with 100 μL Milli-Q water and 200 μL chloroform and centrifuged at 16000g for 5 min at 4 °C. Subsequently, 150 μL of the aqueous solution was centrifugally filtered through a 10-kDa cut-off filter (MRCPRT010, Millipore) to remove proteins. The filtrate was transferred to the glass insert for LC-MS detection.

Sampleprep ID:

SP003127

Sampleprep Summary:

The adipocytes were homogenized in a TissueLyser II (Qiagen) (15 min 30 Hz) at 4 °C. 200 μL of the extracts were mixed with 100 μL Milli-Q water and 200 μL chloroform and centrifuged at 16000g for 5 min at 4 °C. Subsequently, 150 μL of the aqueous solution was centrifugally filtered through a 10-kDa cut-off filter (MRCPRT010, Millipore) to remove proteins. The filtrate was transferred to the glass insert for LC-MS detection.