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MB Sample ID: SA327038
Local Sample ID: | 58_O_HFD.BA |
Subject ID: | SU003125 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP003131 |
Sampleprep Summary: | Colon content was mixed with 5x ACN:H2O solvent and 4 steel beads for subsequent extraction in the bead mill. After centrifugation, 100 µL of the supernatant were mixed with 500 µL methanol:acetonitrile (ACN):water (2:3:1) followed by vortex and sonication. After centrifugation, 100 µL of the supernatant were dried in a SpeedVac™ vacuum concentrator (Eppendorf, Hamburg, Germany). |