Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA327964

Local Sample ID:	D 56-01-9592
Subject ID:	SU003140
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP003146
Sampleprep Summary:	Upon aliquoting the samples into 100 µl Eppendorf tubes, 300 µl of methanol (sourced from Wunstorfer Strasse, Seelze, Germany) was introduced. The tubes underwent thorough mixing with a vortex mixer and were subsequently incubated at –20 °C for 2 hours. After this period, the samples were vortexed again and centrifuged for 15 minutes at 14,000 rpm. The resulting supernatant underwent evaporation at 35–40 °C. To guarantee the analysis's consistency and reliability, a quality control (QC) sample was prepared by combining an equal volume (10 µl) from each individual sample. This QC sample was injected into the system after every 9-10 samples to evaluate the analysis's reproducibility. Before injection, the extracted samples were reconstituted in 250 µl of 0.1% formic acid in deionized water, using Honeywell's LC-MS CHROMASOLV, situated in Wunstorfer Strasse, Seelze, Germany. Following the completion of sample preparation, the supernatant underwent filtration for subsequent LC-MS/MS analysis. This filtration utilized a hydrophilic nylon syringe filter with a pore size of 0.45 µm. The filtered sample was meticulously collected within a specialized insert positioned inside LC glass vials, ensuring its integrity for further analysis.

Sampleprep ID:

SP003146

Sampleprep Summary:

Upon aliquoting the samples into 100 µl Eppendorf tubes, 300 µl of methanol (sourced from Wunstorfer Strasse, Seelze, Germany) was introduced. The tubes underwent thorough mixing with a vortex mixer and were subsequently incubated at –20 °C for 2 hours. After this period, the samples were vortexed again and centrifuged for 15 minutes at 14,000 rpm. The resulting supernatant underwent evaporation at 35–40 °C. To guarantee the analysis's consistency and reliability, a quality control (QC) sample was prepared by combining an equal volume (10 µl) from each individual sample. This QC sample was injected into the system after every 9-10 samples to evaluate the analysis's reproducibility. Before injection, the extracted samples were reconstituted in 250 µl of 0.1% formic acid in deionized water, using Honeywell's LC-MS CHROMASOLV, situated in Wunstorfer Strasse, Seelze, Germany. Following the completion of sample preparation, the supernatant underwent filtration for subsequent LC-MS/MS analysis. This filtration utilized a hydrophilic nylon syringe filter with a pore size of 0.45 µm. The filtered sample was meticulously collected within a specialized insert positioned inside LC glass vials, ensuring its integrity for further analysis.