Return to study ST003031 main page
MB Sample ID: SA328183
Local Sample ID: | DIG011 DP2.Hour 2 plasma |
Subject ID: | SU003145 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP003151 |
Sampleprep Summary: | Plasma (25 μL) was added to 1.5 mL Eppendorf tubes prior to the addition of 10 μL of 1 μM internal standard solution. Next, 750 μL chilled methanol was added and samples were vortexed 30 s before centrifugation at 15,000 × G for 10 min. Subsequently, the supernatant was collected and added to 1.5 mL high performance liquid chromatography (HPLC) amber glass vials, dried, and reconstituted in 100 μL 3:1 acetonitrile: methanol containing 1-cyclohexyl-ureido, 3-dodecanoic acid (CUDA; Sigma-Aldrich, St. Louis, MO, USA) solution. The solution was vortexed 30 s, transferred to microfilter tubes, and centrifuged at 10,000 × G for 3 min prior to transfer to a HPLC vial. |
Sampleprep Protocol Filename: | La_Frano_Lab_Methods_eTRE_v2.pdf |