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MB Sample ID: SA328794
Local Sample ID: | BR_Blank_broth_B3_F |
Subject ID: | SU003150 |
Subject Type: | Bacteria |
Subject Species: | Pseudomonas aeruginosa |
Taxonomy ID: | 287 |
Genotype Strain: | CW41 |
Age Or Age Range: | NA |
Weight Or Weight Range: | NA |
Gender: | Not applicable |
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Sample Preparation:
Sampleprep ID: | SP003156 |
Sampleprep Summary: | Each sample (25 µL) was added to 100 µL of pre-chilled methanol containing the internal standards (CHAPS, CAPS, TRIS and PIPES) at 1 µM. This mixture was vortexed, and subsequently centrifuged at 14800 x g and 4°C for 10 min .The final supernatant samples containing the extracted extracellular metabolites were stored at -80°C until LC-MS analysis was performed (Figure S2B). |
Processing Method: | IDEOM |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |