Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA330508

Local Sample ID:	PR_150
Subject ID:	SU003160
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

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Sample Preparation:

Sampleprep ID:	SP003166
Sampleprep Summary:	50 mg of pulverized tissue was extracted using 200 ul each of hexane, chloroform, methylene chloride, and methanol. The mixture was sonicated for 10 minutes each time after adding organic solvent, then centrifuged for 10 min at 11,000 x g at 4°C, and dried in a vacuum centrifuge. The dried extract was then subjected to derivatization by adding 40 μl of methoxyamine hydrochloride in pyridine (20 mg/ml) and incubated for 1.5h at 37 °C. Next, in the second derivatization step, 90 μl of N-Trimethylsilyl-N-methyl triﬂuoroacetamide was added, and samples were incubated at 37 °C for another 30 min. After derivatization, samples were immediately subjected to a GC/MS analysis.

Sampleprep ID:

SP003166

Sampleprep Summary:

50 mg of pulverized tissue was extracted using 200 ul each of hexane, chloroform, methylene chloride, and methanol. The mixture was sonicated for 10 minutes each time after adding organic solvent, then centrifuged for 10 min at 11,000 x g at 4°C, and dried in a vacuum centrifuge. The dried extract was then subjected to derivatization by adding 40 μl of methoxyamine hydrochloride in pyridine (20 mg/ml) and incubated for 1.5h at 37 °C. Next, in the second derivatization step, 90 μl of N-Trimethylsilyl-N-methyl triﬂuoroacetamide was added, and samples were incubated at 37 °C for another 30 min. After derivatization, samples were immediately subjected to a GC/MS analysis.